9 best lab cell culture flasks

Laboratory cell culture flasks are essential pieces of glassware used in the field of cell biology and microbiology for the cultivation and growth of cells.These flasks are designed to create a controlled environment that supports the growth and maintenance of various cell types, including mammalian cells, bacterial cultures, and yeast. Here are some key points about lab cell culture flasks:

Purpose: Cell culture flasks are used to provide a sterile and controlled environment for the in vitro (outside of the living organism) growth and maintenance of cells. This allows researchers to study cellular behavior, conduct experiments, and produce cell-based products.
Design: Cell culture flasks typically have a flat, rectangular, or triangular shape with a screw-on or plug-style cap. The flask's design facilitates the growth of a monolayer of adherent cells on the inner surface.
Materials: These flasks are typically made from high-quality, optically clear borosilicate glass or transparent plastics like polystyrene. The choice of material depends on the specific requirements of the cell culture, such as the compatibility with cell types and the ability to withstand sterilization methods.
Sterility: Maintaining a sterile environment is crucial in cell culture. Cell culture flasks are sterilized before use and sealed with a cap or plug to prevent contamination. They are also often designed for single-use to minimize the risk of cross-contamination between cultures.
Surface Treatments: Some cell culture flasks have surface treatments to enhance cell adhesion, growth, and differentiation. For example, tissue culture-treated flasks have a treated surface to support the attachment and growth of adherent cell lines.
Ventilation: Some flasks may include filters or vented caps to allow the exchange of gases (e.g., oxygen and carbon dioxide) while preventing contamination.
Sizes and Capacities: Cell culture flasks come in various sizes and capacities, ranging from small 25 cm^2 flasks for small-scale experiments to larger 175 cm^2 or more flasks for large-scale cell culture production.
Accessories: Accessories such as cell scrapers, pipettes, and pipette controllers are commonly used in conjunction with cell culture flasks for various cell culture techniques and procedures.
Applications: Cell culture flasks are widely used in research laboratories, biotechnology companies, pharmaceutical companies, and academic institutions for applications like drug discovery, vaccine development, tissue engineering, and fundamental cellular biology studies.

It's essential to follow proper aseptic techniques when working with cell culture flasks to maintain cell health and prevent contamination. Additionally, specific protocols and media are used depending on the type of cells being cultured and the research objectives.

Below you can find our editor's choice of the best lab cell culture flasks on the market

250mL Treated Tissue Culture Flasks with Vent Cap, Sterile, 100/pk

BlokCell

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Product description

These tissue culture flasks come with vented caps with breathable membranes. The shape allows for easy stackability and organization. Engraved graduations on both side allow for precise measurements with a frosted writing area for traceability.

Treated
Sterilized by Gamma Irradiation
Non-pyrogenic
Certified Non-PyroDNase/RNase free
High clarity medical grade polystyrene material

SPL Cell Culture Flask with Vented with Filter Cap, Tissue Culture Treated, Sterile, 25 cm², Clear, 5 per Pack, Case of 200

SPL Life Sciences

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Product description

SPL Tissue Culture Flask with Filter Cap, Tissue Culture 25 cm2

Culture flasks of SPL Life Sciences are ergonomically designed for easy handling, while achieving minimal contamination during cell culture. Short, wide and angled neck design enables easy access of serological pipettes and cell scrapers.

Features:

▪ Ergonomic design to facilitate easy handling and minimize contamination

▪ Short & wide neck with angled design allow easy access

▪ Excellent stackability

▪ Filter caps are available for all flask models

▪ Cell growth area ranging 25 cm²

▪ Non – pyrogenic

▪ Non – cytotoxic

▪ DNase / RNase – free

▪ Human DNA – free

Specification:

- Material: Polystyrene PS

- Cap Type: Filter

- Growth Area (cm²): 25.00

- Working Volume (ml): 7.00

- Total Volume (ml): 60.00

- Surface Treatment: Yes

- Sterile: Yes

- Packaging: Sleeve of 5 (200 Flask x Box)

What is in the Box?

200 x SPL Culture Flask 25 cm2 / sleeve of 5

Ergonomic design to facilitate easy handling and minimize contamination
Short & wide neck with angled design allow easy access
Excellent stackability
Non – pyrogenic, Non – cytotoxic, DNase / RNase – free, Human DNA – free

Sarstedt T-175 Cell Culture Flask with Standard TC Surface and Vented Cap, TC Tested

Sarstedt

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Product description

Flask, cell culture, T-175, polystyrene, standard TC surface for adherent cells, canted neck, red cap with filter, printed with expiration date and lot number, TC Tested (sterile, non-pyrogenic/endotoxin-free, non-cytotoxic, DNase-/RNase-/DNA-free), 5 pcs./zip seal bag

Standard TC growth surface for adherent cells, color coded with red cap
Canted neck and flask design enable accessibility to all corners with pipettes and scrapers
Vented cap with 0.2Âµm pore size filter ensures constant, sterile gas exchange
Each flask labeled with lot number and expiration date; flasks packaged in resealable zip bags
TC Tested: sterile, non-cytotoxic, and free of DNA, DNase, RNase, and pyrogens

Tissue Culture Flasks, Cell Culture T182 Flask, Plug Cap, 600ml, Growth Area: 182.0com2, 5 per Sleeve, 40 Flasks/Unit

Olympus Plastics

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Product description

Olympus Cell and Tissue Culture Flasks are offered in 12.5, 25, 75, 182 cm2 sizes and are available tissue culture treated or non-treated. Distortion free, completely flat growth surfaces provide even and consistent cell growth for optimal proliferation of cells. Flasks are graduated with a frosted writing area and feature a wide, angled neck design that offers 100% access for washing and retrieving cells. All flasks are available with your choice of vented or plug seal caps. Vented caps feature a 0.22µm hydrophobic membrane filter that allows gas exchange and excludes bacterial and fungal contaminates. Plug seal caps provide an air tight seal or allow gas exchange with a one quarter turn of the cap. The pronounced rim allows for easy stacking and molded spacers on the base create venting gaps that allow heat and gas exchange between stacked units to speed up acclimation to 37ºC. All flasks are sterilized by gamma irradiation and certified RNase, DNase and Pyrogen Free.

100% angled neck access - better for washing cells and scraping cells
Optically clear, USP VI crystal class virgin polystyrene for distortion free microscopy
0.22um hydrophobic vented filter cap or 2-position plug caps available
Striation free, completely flat bases for even and consistent cell growth
Packaged in user-friendly resealable zip closure sleeves to maintain sterility

Sterile Vented Plastic Erlenmeyer Flasks for Cell Culture and Fermentation, 1L, Autoclavable Polycarbonate (PC), 53mm (53B) VersaCap with 0.2um PTFE Vent, Baffled Bottom (Pack of 6)

Foxx Life Sciences

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Product description

Foxx Life Sciences EZclear Erlenmeyer Shaker Flasks are molded from virgin, optically clear, non-leaching polycarbonate resin. Our flasks retain clarity after gamma sterilization, and comply with stringent and USP requirements. Sterile flasks are additionally certified as non-pyrogenic and DNase/RNase-free, making them ideal for cell cultures, microbial cultures, plants, media preparation, storage, and all other applications. They also fit all standard shaking incubator clamps and can be placed on any platform. Foxx Life Sciences EZclear Flasks are available with a flat or baffled base for increased oxygenation and mixing. Flasks are compatible with all 53mm VersaCap technology and adapter inserts, enabling easy customization of top connections. Vented VersaCap with a 0.2µm hydrophobic membrane can be purchased with the unit, or separately as an accessory. All Flasks and VersaCap are autoclavable at 15 PSI and 121˚C for 20 minutes.

Flasks and VersaCaps are autoclavable
Vented VersaCaps available
Sterility tested to SAL 10^-6
Molded in graduations
Sterile flasks are DNase/RNase-free and non-pyrogenic

Product features

Developed with You in Mind

Foxx Life Sciences’ Flasks are available with a flat or baffled base for increased oxygenation and mixing. Flasks are compatible with all 53mm VersaCap technology and adapter inserts, enabling easy customization of top connections. Vented VersaCap with a 0.2µm hydrophobic membrane can be purchased with the unit, or separately as an accessory. All Flasks and VersaCap are autoclavable at 15 PSI and 121˚C for 20 minutes.

Corning 10030 Polystyrene 560mL Rectangular CellBIND Surface Treated HYPERFlask M Cell Culture Vessel with Bar Code, Individually Double Bagged (Case of 4)

Corning

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Product description

This Corning high yield performance flask manual (HYPERFlask M) cell culture vessel is specifically designed for manual use. Originally, the HYPERFlask cell culture vessel was developed for automation. Due to its popularity for use on the bench top, Corning has redesigned the HYPERFlask M vessel with new features. It has an innovative 10 layer design of interconnected gas permeable polystyrene growth surfaces, and is Corning CellBIND Surface treated for superior cell attachment and growth, allowing a ten-fold higher cell yield that increases productivity and capacity. It has a new ergonomic cap with a smooth texture on the liner that allows more ergonomic closing and opening. The new adaptor grid allows for faster filling and emptying while reducing foam generation. Reduce processing time and incubator storage space by handling one flask compared to 10 traditional 175sq cm flasks. Each individual flask is traceable by a serial number that can be read by the unaided eye or by a handheld bar code reader. It is made with USP Class VI materials molded and assembled in a Certified Class 100,000 clean room. Gamma irradiated to sterility assurance level (SAL) 10-6 and individually double bagged four to a case in clean room packaging. Ungraduated. Sterile. Material: Polystyrene. Cell Growth Area: 1720sq cm. Flask Shape: Rectangular. Neck Style: Straight. Surface Treatment: Corning CellBIND Surface. Cap Style: Flat Top. Cap Color: Orange. Cap Material: High Density Polyethylene. Recommended Medium Volume: 560mL. Maximum Working Volume: 560mL. Corning #: 10030.

High yield performance flask is specifically designed for manual use with many new features including an ergonomic HDPE cap and adaptor grid
Rectangular shaped flask has a straight neck style, with a maximum working volume of 560mL and a cell growth area of 1720sq cm
Features 10 interconnected CellBIND Surface treated gas permeable polystyrene growth surfaces for superior cell attachment and growth
Each individual flask is traceable by a serial number that can be read by the unaided eye or by a handheld bar code reader

Nest Scientific 708003 TC-Treated Cell Culture Flask, Vent Cap, Sterile, 649.52 Cubic cm, Polystyrene, Clear (Pack of 100)

NEST ABOVE

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Product description

Nest cell culture flasks are available in cell growth areas ranging from 25cm2 to 225cm2. Tissue culture treated or non-treated flasks available with a vent cap or plug seal cap to meet your needs. Vent caps with 0.22 um hydrophobic filters for gas exchange.

Angled neck for easy access
Packaged in Sterile, zip-sealable bags
High clarity, 100% virgin polystyrene
Frosted writing and clear graduations
Notched bottom for slip free stacking

Kimax Long Neck Culture Flask with Capacity Scale, 100ml Subdivision, 38mm Neck OD, 2000ml Capacity (Case of 12)

Kimax

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Product description

Culture flask with an extended, thick-walled neck that accepts a metal cap, plastic closure, foam plug, or cotton plug for cell culture applications
Designed from ASTM Specification E1404, Type IV, Class I requirements
Made of 33 expansion, low-extractable borosilicate glass that meets USP Type I and ASTM E438, Type I, Class A requirements

NEST Scientific 230124 Cell Culture Chamber Slide, 4 Well with Glass Slide, White, 2.13 cm² Cell Growth Area, 0.5-1.3mL Working Volume (2)

NEST SCIENTIFIC USA

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Product description

Nest Cell Culture Chamber Slides are designed for various cell-based experiments and are also useful for virus, toxicity tests and immunocytological experiments. Cell Culture Chamber Slides provide removable chambers that enable cell growth directly on the microscopic slide, convenient for staining and microscopic examination without cell transfer. Chambers with various well numbers and colors are available for different applications.